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Ukraine on Saturday accused Russian forces of gunning down 24 civilians including children in an attack on a road convoy near the recently recaptured town of Kupiansk.
On Friday, Ukrainian troops had shown AFP reporters a group of vehicles riddled with bullet holes and several corpses in civilian clothes outside the village of Kyrylivka, a short distance east of Kupiansk.
Kharkiv regional governor Oleg Synegubov put the preliminary death toll at 24 including, he said, a pregnant woman and 13 children, alleging: "Russians fired on the civilians at close range."
The Kharkiv region prosecutor's office said: "On September 30 prosecutors, investigators of the Security Service and the police discovered a convoy of seven cars that had been shot.
"The car queue was shot by the Russian army on September 25, when civilians were trying to evacuate," the statement alleged.
"Two cars have burnt completely with children and their parents inside -- they burnt alive," it said.
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Ukrainian officials have launched an investigation and allege that the Russian forces that were driven out of Kyrylivka last Sunday were behind the war's latest apparent massacre.
"The occupiers are being defeated on the battlefield and desperately respond by killing civilians," said the head of the Security Service of Ukraine, Vasyl Malyuk.
"We will find everyone and hold them accountable for committed atrocities," he said. "Several people had managed to escape from Hell. Now they're testifying to the investigators."
Governor Synegubov's spokeswoman told AFP the site of the killings was still unsafe for investigators, being a short distance from the still active frontline between Ukrainian and Russian forces.
On Friday, AFP reporters counted the bodies of at least 11 civilians in the wrecked cars, some burned beyond recognition.
Ukrainian troops operating nearby said they had found the dead on an exposed section of road alongside a rail track, as defeated Russian forces retreated from the area.
On Friday the bodies remained where they had died in and around the vehicles on a road running southeast from Kyrylivka 70 kilometres (43 miles) east of Kharkiv, in the direction of the Russian retreat.
A small van or minibus was completely burnt out, with the carbonised remains of four people within. At least one of these corpses appeared small enough to have been a child.
"The vehicles were moving all together as a civilian convoy. We can see that there is almost no distance between the vehicles," said "Filya", a Ukrainian soldier who guided AFP to the scene.
"And obviously they were attacked by gunfire because you can see the bullet holes ... One car was set on fire and it's impossible to recognise the people without an expert," he said.
Russia's February invasion allowed Moscow to seize a swathe of northern and eastern Ukraine, but this month a lightning Ukrainian counteroffensive in the Kharkiv region has driven them back.
Russian occupations forces have been accused of brutalising and murdering civilians several times during the seven-month-old war, and have hit cities with deadly missile strikes.